Recombinant Human Caspase 1, Apoptosis-Related Cysteine Peptidase (interleukin 1, beta, convertase), His-tagged

• Cat.No.: CASP1-1639H
• Product Overview: Recombinant active human CASP1 containing N-term His-tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: CASP1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. CASP1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active CASP1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of CASP1 activation and the role of CASP1 in apoptosis are poorly understood.
• Biological Activity: > 5000 units/mg
• Molecular Weight: large (20 kD) and small (10 kD) subunitsk
• Form: Lyophilized powder.
• Appearance: Lyophilized powder
• Reconstitution: Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
• Storage: The lyophilized CASP1 is stable for 1 year at -70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at -70°C. Avoid multiple freeze/thaw cycles as activity might decrease.
• Pathways: Amyotrophic lateral sclerosis (ALS); Androgen Receptor Signaling Pathway; Apoptosis; Caspase cascade in apoptosis; Cellular roles of Anthrax toxin; Cytokine Signaling in Immune system; Cytosolic DNA-sensing pathway; Direct p53 effectors; IFN-gamma pathway; IL1-mediated signaling events; Immune System; Inflammasomes; Influenza A; Innate Immunity Signaling; Interleukin-1 processing

Gene Information

• Gene Name: CASP1 caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) [ Homo sapiens ]
• Official Symbol: CASP1
• Synonyms: CASP1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); ICE; P45; IL1BC; caspase-1; IL-1BC; IL1B-convertase; CASP1 nirs variant 1; IL-1 beta-converting enzyme; interleukin 1-B converting enzyme; Caspase-1; CASP-1; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; EC 3.4.22.36; OTTHUMP00000045728; OTTHUMP00000232029; OTTHUMP00000232030; OTTHUMP00000232031; OTTHUMP00000232032; OTTHUMP00000232033; OTTHUMP00000232034; OTTHUMP00000232036
• Gene ID: 834
• mRNA Refseq: NM_033292
• Protein Refseq: NP_150634
• MIM: 147678
• UniProt ID: P29466
• Chromosome Location: 11q23
• Function: cysteine-type endopeptidase activity; caspase activator activity; peptidase activity; protein binding