Recombinant Human Poly (ADP-ribose) Polymerase 1
Cat.No. : | PARP1-1658H |
Product Overview : | Recombinant human PARP1 expressed by Baculovirus is a 116.0 kDa homodimeric protein. |
- Specification
- Gene Information
- Related Products
Cat. No. : | PARP1-1658H |
Description : | PARP1 also known as NAD ADP-ribosyltransferase 1 or poly [ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 Enzyme, is highly purified and enzymatically active human PARP1, expressed in a baculovirus expression system. Upon addition of NAD+ and cofactors the PARP1 is automodified by the addition of ADP-ribose monomers to form poly (ADP-ribose) polymer. |
Biological Activity : | 1000 U/vial, specific activity = 20000 U/mg PARP1. 1U =10 fmol ADP-ribose incorporated into 5 μg immobilized histone in 30 min at room temperature. Note: Activity measurements are approximate values. |
Molecular Weight : | 116.0 kDa |
Source : | Baculovirus |
Species : | Human |
Form : | Lyophilized solid. |
Appearance : | Liquid |
Reconstitution : | Spin tube in a microfuge for 15 sec to sediment lyophilized material. Carefully open the vial and add 100 μL dH₂O. Vortex gently for 20 sec (avoid air bubbles). Let stand for 5 min. Carefully triturate the sample 10-times using a pipetman (avoid air bubbles). Spin briefly in microfuge to consolidate. Upon reconstitution with 100 μL dH20, the final concentrations are as follows: 0.5 mg/mL PARP1 enzyme, 20 mM Tris, pH 8, 0.3 M NaCl, 0.1 mM EDTA, 1 mM DTT, plus lyophilization stabilizers. |
Purity : | ≥95% by SDS-PAGE |
Storage : | Store reconstituted solution at -70°C. Avoid multiple freeze-thaw cycles. There is loss of PARP enzymatic activity upon each free/thaw cycle. It is suggested to aliquot the reconstituted enzyme into multiple tubes and freeze at -70°C. Alternatively, add glycerol at 1:1 vol/vol to the reconstituted PARP1, mix gently by trituration, and store at -20°C (do not store in a frost-free freezer!) for up to 6 months. |
Gene Name : | PARP1 poly (ADP-ribose) polymerase 1 [ Homo sapiens ] |
Official Symbol : | PARP1 |
Synonyms : | PARP1; poly (ADP-ribose) polymerase 1; PARP; PPOL; ADPRT; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; poly [ADP-ribose] polymerase 1; OTTHUMP00000035663; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly[ADP-ribose] synthase 1; poly(ADP-ribosyl)transferase; ADP-ribosyltransferase NAD(+); NAD(+) ADP-ribosyltransferase 1; poly (ADP-ribose) polymerase family, member 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); EC=2.4.2.30 |
Gene ID : | 142 |
mRNA Refseq : | NM_001618 |
Protein Refseq : | NP_001609 |
MIM : | 173870 |
UniProt ID : | P09874 |
Chromosome Location : | 1q41-q42 |
Pathway : | BER complex; BER complex; Base excision repair; Caspase cascade in apoptosis; FAS pathway and Stress induction of HSP regulation; Notch-mediated HES/HEY network |
Function : | DNA binding; NAD binding; NAD+ ADP-ribosyltransferase activity; metal ion binding; protein N-terminus binding; protein binding; transcription factor binding; transferase activity, transferring glycosyl groups; zinc ion binding |
Products Types
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ZNF101-434H | Recombinant Human PARP1 Protein, MYC/DDK-tagged | +Inquiry |
PARP1-075H | Recombinant Human PARP1 Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
PARP1-2615H | Recombinant Human PARP1 protein(61-370 aa), C-His-tagged | +Inquiry |
◆ Lysates | ||
PARP1-514MCL | Recombinant Mouse PARP1 cell lysate | +Inquiry |
PARP1-710HCL | Recombinant Human PARP1 cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-1794 | PARP1 Colorimetric Assay Kit | +Inquiry |
Kit-1793 | PARP1 Chemiluminescent Assay Kit (384-well) | +Inquiry |
Kit-1792 | PARP1 Chemiluminescent Assay Kit | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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