Recombinant Rat Interleukin 18

• Cat.No.: Il18-522R
• Product Overview: Recombinant rat IL-18 is a single,non-glycosylated polypeptide containing 158 amino acids and having amolecular weight of 18.3 kDa.

Specification

• Species: Rat
• Source: E.coli
• Tag: Non
• Description: Interleukin-18also known as IL18 is a protein which in humans is encoded by the IL18 gene.The protein encoded by this gene is a proinflammatory cytokine.
• Biological Activity: The Rat IL-18 isfully biologically active when compared to standard. The ED50 asdetermined by the dose-dependent stimulation of IFN- gamma by murine lymphnode cells, stimulated with sub optimal dose of ConA (0.75 µg/ml) is 12ng/ml.
• Molecular Weight: 18.3 kDa
• Form: Lyophilized (from 1mg/ml in 0.4X PBS)
• Appearance: Lyophilized protein
• Endotoxin Level: <0.1 ng/µg
• Reconstitution: Reconstitute inwater to a concentration of 0.1-1.0 µg/µl. Gentle pipetting to mix it well.The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
• Purity: >95% by SDS-PAGEand HPLC analysis
• Storage: Thelyophilized IL-18 is best-stored desiccated below 0°C. Reconstituted IL-18飥?should be stored at working aliquots at -20°C.
• Pathways: African trypanosomiasis;Cytokine-cytokine receptor interaction; Cytosolic DNA-sensing pathway;Hypertrophy Model; Influenza A; Legionellosis; Malaria; NOD-like receptorsignaling pathway; Rheumatoid arthritis; Salmonella infection; Tuberculosis

Gene Information

• Gene Name: Il18 interleukin 18 [ Rattus norvegicus]
• Official Symbol: Il18
• Synonyms: Il18; interleukin 18; IL-18;IGIF; interleukin-18; IL-1 gamma; interleukin-1 gamma; IFN-gamma-inducingfactor; interferon gamma-inducing factor; interleukin 18(interferon-gamma-inducing factor); MGC12320; IL1F4; iboctadekin; IL-1g;Interferon gamma-inducing factor; Iboctadekin; Interleukin-1 gamma
• Gene ID: 29197
• mRNA Refseq: NM_019165
• Protein Refseq: NP_062038
• UniProt ID: P97636
• Chromosome Location: 8q23
• Function: cytokine activity; interleukin-18receptor binding; protein binding

Q&As

What are the difference between IL18-01HG, IL18-153HG and IL18-500H. (sequence, biological activity, QC, or what do you propose to buy)

IL18-01HG: Sequence: a.a.37-193 Endotoxin: <20 EU/mg Bio-activity: >5.0 x 10^4 IU/mg QC: Activity test, SDS-PAGE, LAL, SEC-HPLC IL18-153HG: Protein length: Tyr37-Asp193 AA Sequence: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDIIFFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIMFTVQNEDVD Bio-activity: 1.0*10^6IU Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha. QC: Activity test, SDS-PAGE, LAL IL18-500H Recombinant Human IL18 will be custom produced upon order. All the sequence, purity, activity, and QC procedures will be optional and decided by our customer.