Active Recombinant Rhesus / Cynomolgus IL18 protein, His-tagged

• Cat.No.: IL18-334R
• Product Overview: Recombinant Rhesus / Cynomolgus IL18 protein(Q9BGI5)(Tyr37-Asp193) was expressed in E.coli with a polyhistidine tag at the C-terminus.

Specification

• Species: Rhesus / Cynomolgus
• Source: E.coli
• Tag: His
• Protein Length: 37-193 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Immobilized Recombinant Human IL18BP Protein (Fc Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus / Rhesus IL-18 Protein (Cleavage & His Tag), the EC50 is 15-45 ng/mL.
• Molecular Mass: The recombinant Cynomolgus / Rhesus IL18 comprises 163 amino acids and has a predicted molecular mass of 19.1 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: ≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Official Symbol: IL18

Q&As

What are the difference between IL18-01HG, IL18-153HG and IL18-500H. (sequence, biological activity, QC, or what do you propose to buy)

IL18-01HG: Sequence: a.a.37-193 Endotoxin: <20 EU/mg Bio-activity: >5.0 x 10^4 IU/mg QC: Activity test, SDS-PAGE, LAL, SEC-HPLC IL18-153HG: Protein length: Tyr37-Asp193 AA Sequence: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDIIFFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIMFTVQNEDVD Bio-activity: 1.0*10^6IU Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha. QC: Activity test, SDS-PAGE, LAL IL18-500H Recombinant Human IL18 will be custom produced upon order. All the sequence, purity, activity, and QC procedures will be optional and decided by our customer.