Recombinant Mouse Lipopolysaccharide Binding Protein

• Cat.No.: Lbp-4928M
• Product Overview: Recombinant mouse Lbp was expressed by baculovirus in CHO cells and having a molecular mass of 64.0 kDa.

Specification

• Cat. No.: Lbp-4928M
• Description: Natural LBP is a 64 kDa glycoprotein produced in liver. It binds at lipid A of LPS with high affinity (10-9M) and reduced the cellular LPS effects at CD14+ cells (IL1ß, IL6, TNFα). It acts as opsonin for GRAM negative cells, LPS, neutrophils and granulocytes.
• Biological Activity: Up to 2 µg/ ml LBP mediates binding of FITC-LPS (0.5µg/ml) to CD14+CHO transfectants (2x106/ml)
• Molecular Weight: 64.0 kDa
• Source: CHO cells
• Species: Mouse
• Form: Lyophilized from a concentrated protein solution (1 mg/ml) containing PBS, pH 7.2.
• Appearance: Lyophilized protein
• Endotoxin Level: <0.01 ng/μg
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in 10 µl of ultra-pure distilled water. This solution can then be diluted into other aqueous buffers and stored at -20°C or -70°C for future use.
• Purity: SDS-PAGE analyses
• Storage: The lyophilized protein is best-stored desiccated below 0°C. Reconstituted mouse-LBP should be stored in working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (10% BSA). Repeated freezing and thawing should be avoided.
• Pathways: Toll-like receptor signaling pathway

Gene Information

• Gene Name: Lbp lipopolysaccharide binding protein [ Mus musculus ]
• Official Symbol: Lbp
• Synonyms: Lbp; lipopolysaccharide binding protein; Ly88; Bpifd2; lipopolysaccharide-binding protein; OTTMUSP00000016997; MGC22233; LPS-binding protein
• Gene ID: 16803
• mRNA Refseq: NM_008489
• Protein Refseq: NP_032515
• UniProt ID: Q61805
• Chromosome Location: 2 H1; 2 83.0 cM
• Function: lipid binding; lipopolysaccharide binding; lipoteichoic acid binding; receptor binding