|Product Overview :||Recombinant Human BMP2 protein was expressed in Escherichia coli.|
|Description :||Bone Morphogenetic Protein 2 is one of the BMPs, some of which belong to the TGF-beta superfamily (BMP2-7). There are more than thirteen BMPs have been discovered nowadays and they are involved in inducing cartilage and bone formation. BMP-2 is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. It is a potent osteoinductive cytokine and plays its role in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. As it shown to stimulate the production of bone, the recombinant human BMP-2 is available for orthopaedic usage and dentistry. The functional form of BMP-2 is a disulflde-linked homodimer and about 26 kDa The precursor of BMP-2 is 259 a.a., containing the 23 a.a. N-terminal signal sequence which will be cleaved by a furin-type protease to form the mature peptide.|
|Form :||Lyophilized from a 0.2μm filtered concentrated solution containing 10 mM sodium citrate pH 3.5.|
|Bio-activity :||Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 200 ng/ml, corresponding to a specific activity of > 5.0 × 10³ IU/mg.|
|Molecular Mass :||Approximately 26.0 kDa, a homodimeric protein consisting of two 115 amino acid non-glycosylated polypeptide chains.|
|Protein length :||115|
|AA Sequence :||MQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR|
|Endotoxin :||Less than 1 EU/µg of rHuBMP-2 as determined by LAL method.|
|Purity :||>95% by SDS-PAGE and HPLC analysis.|
|Storage :||Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.|
|Reconstitution :||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. Do not reconstitute in PBS or cell culture media.|
|SDS-PAGE of BMP2-01H :||
Controlled release of injectable liposomal in situ gel loaded with recombinant human bone morphogenetic protein-2 for the repair of alveolar bone clefts in rabbits.
Biofunctionalizing devitalized bone allografts through polymer‐mediated short and long term growth factor delivery.
Bone Morphogenetic Protein-2 Adsorption onto Poly-?-caprolactone Better Preserves Bioactivity In Vitro and Produces More Bone In Vivo than Conjugation Under Clinically Relevant Loading Scenarios.
Depot injectable biodegradable nanoparticles loaded with recombinant human bone morphogenetic protein-2: preparation, characterization, and in vivo evaluation.
Dual Delivery of BMP2 and VEGF from a modular polycaprolactone scaffold for the treatment of large bone.
Dual Delivery of BMP2 and VEGF from a polycaprolactone/collagen sponge construct to increase bone growth in ectopic sites for flap prefabrication.
Dual Delivery of EPO and BMP2 from a Novel Modular Poly-?-Caprolactone Construct to Increase the Bone Formation in Prefabricated Bone Flaps.
Bone tissue engineering in the greater omentum is enhanced by a periosteal transplant in a miniature pig model
|Gene Name :||BMP2|
|Official Symbol :||BMP2|
|Synonyms :||BMP2; bone morphogenetic protein 2; BMP2A; BMP-2A; bone morphogenetic protein 2A; BDA2;|
|Gene ID :||650|
|mRNA Refseq :||NM_001200|
|Protein Refseq :||NP_001191|
|UniProt ID :||P12643|