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Recombinant Human Mitogen-Activated Protein Kinase Kinase Kinase 8, GST-tagged

Cat.No. : MAP3K8-988H
Product Overview : Recombinant human MAP3K8 (30-397) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
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  • Gene Information
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Cat. No. : MAP3K8-988H
Description : MAP3K8 is an oncogene that can activate both the MAP kinase and JNK kinase pathways. MAP3K8 activates IκB kinases and induces the nuclear production of NF-κB. C-terminal catalytic domain of KSR2 associates with MAP3K8 and KSR2 can negatively regulates the kinase activity of MAP3K8 in vitro. Co-transfection of KSR2 with MAP3K8 in cells leads to reduced MAP3K8-mediated ERK activation and MAP3K8-induced IL8 production in a dose-dependent manner. MAP3K8 is one of the MAP kinase kinase kinases that regulates the ERK1/ERK2 pathway in response to IL-1. Blockage of expression of MAP3K8 results in failure of IL-1 to induce an increase in IL-8 and MIP-1betamRNA levels.
Applications : Kinase Assay; Western Blot
Molecular Weight : 70 kDa
Expression System : Sf9 insect cells using baculovirus
Species : Human
Form : Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol.
Purity : > 90 %
Concentration : 0.2 ug/ul
Sequences : 30-397
Storage : Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Pathways : Adaptive Immunity Signaling; CD28 co-stimulation; Costimulation by the CD28 family; Immune System; Insulin Signaling; Interleukin-1 signaling; MAPK signaling pathway; Signaling by Interleukins; T cell receptor signaling pathway; Toll-like receptor signaling pathway; TNF-alpha/NF-kB Signaling Pathway; TCR signaling in naive CD8+ T cells; TCR signaling in naive CD4+ T cells; Role of Calcineurin-dependent NFAT signaling in lymphocytes; CD28 dependent PI3K/Akt signaling
Gene Name : MAP3K8 mitogen-activated protein kinase kinase kinase 8 [ Homo sapiens ]
Official Symbol : MAO3K8
Synonyms : MAP3K8; mitogen-activated protein kinase kinase kinase 8; EST; COT; MEKK8; Tpl-2; c-COT; Proto-oncogene c-Cot; Serine/threonine-protein kinase cot; Tumor progression locus 2; ESTF; Cancer Osaka thyroid oncogene; TPL2; FLJ10486; cot (cancer Osaka thyroid) oncogene; Ewing sarcoma transformant; proto-oncogene serine/threoine protein kinase; tumor progression locus-2; TPL-2; EC 2.7.11.25; OTTHUMP00000019392; OTTHUMP00000019393
Gene ID : 1326
mRNA Refseq : NM_005204
Protein Refseq : NP_005195
MIM : 191195
UniProt ID : P41279
Chromosome Location : 10p11.23
Function : ATP binding; MAP kinase kinase kinase activity; magnesium ion binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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