"BRAF" Related Products

Recombinant Human V-Raf Murine Sarcoma Viral Oncogene Homolog B1, GST-tagged

Cat. No.: BRAF-994H
Product Overview: Recombinant human BRAF (416-end; V599E) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Description: BRAF is a member of the RAF family that is activated by members of the Ras family upon growth factor-induced stimulation. Active Ras can induce heterodimerization of cRaf and BRAF and this may explain the observed cooperativity of cRaf and BRaf in cells responding to growth factor signals. Activating mutations in the BRAF gene are present in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations result in a valine to glutamic acid change at residue 599 within the activation segment of B-RAF.
Applications: Kinase Assay; Western Blot
Molecular Weight: 63 kDa
Expression System: Sf9 insect cells using baculovirus
Species: Human
Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol.
Specific Activity: 98 nmol/min/mg
Purity: > 80 %
Concentration: 0.1 ug/ul
Sequences: 416-end
Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Pathways: Acute myeloid leukemia; Bladder cancer; Chemokine signaling pathway; Chronic myeloid leukemia; Colorectal cancer; Endometrial cancer; ErbB signaling pathway; Focal adhesion; Glioma; Insulin signaling pathway; Long-term depression; Long-term potentiation; MAPK signaling pathway; Melanoma; Natural killer cell mediated cytotoxicity; Neurotrophin signaling pathway
Gene Name: BRAF v-raf murine sarcoma viral oncogene homolog B1 [ Homo sapiens ]
Official Symbol: BRAF
Synonyms: BRAF; v-raf murine sarcoma viral oncogene homolog B1; NS7; BRAF1; RAFB1; B-RAF1; FLJ95109; MGC126806; MGC138284; serine/threonine-protein kinase B-raf; p94; 94 kDa B-raf protein; proto-oncogene B-Raf; murine sarcoma viral (v-raf) oncogene homolog B1; B-Raf proto-oncogene serine/threonine-protein kinase (p94); Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; EC 2.7.11.1; OTTHUMP00000212184
Gene ID: 673
mRNA Refseq: NM_004333
Protein Refseq: NP_004324
MIM: 164757
UniProt ID: P15056
Chromosome Location: 7q34
Function: mitogen-activated protein kinase kinase binding; MAP kinase kinase kinase activity; protein heterodimerization activity; protein serine/threonine kinase activity; protein tyrosine kinase activity; receptor signaling protein activity; ATP binding; metal ion binding; nucleotide binding; protein binding; transferase activity; zinc ion binding

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