Active Recombinant Human LIMK1, GST-tagged
Cat.No. : | LIMK1-1029H |
Product Overview : | Recombinant human LIMK1 (285-638) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. |
- Specification
- Gene Information
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Description : | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
Source : | Sf9 insect cells using baculovirus |
Species : | Human |
Tag : | GST |
Form : | Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
Bio-activity : | 8 nmol/min/mg |
Molecular Mass : | ~65 kDa |
Purity : | >95% |
Applications : | Kinase Assay, Western Blot |
Stability : | 1 yr at -70°C from date of shipment. |
Storage : | Store product at –70ºC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
Concentration : | 0.1ug/ul |
Gene Name : | LIMK1 LIM domain kinase 1 [ Homo sapiens (human) ] |
Official Symbol : | LIMK1 |
Synonyms : | LIMK1; LIM domain kinase 1; LIMK; LIM motif-containing protein kinase; LIMK-1; NP_001191355.1; EC 2.7.11.; NP_002305.1 |
Gene ID : | 3984 |
mRNA Refseq : | NM_002314 |
Protein Refseq : | NP_002305 |
MIM : | 601329 |
UniProt ID : | P53667 |
Chromosome Location : | 7q11.23 |
Pathway : | CDC42 signaling events; CXCR4-mediated signaling events; EPHB-mediated forward signaling; Fc gamma R-mediated phagocytosis |
Function : | ATP binding; heat shock protein binding; protein heterodimerization activity; protein serine/threonine kinase activity |
Products Types
◆ Recombinant Protein | ||
LIMK1-39H | Recombinant Human LIMK1 Protein (285-638), N-GST tagged | +Inquiry |
LIMK1-5085M | Recombinant Mouse LIMK1 Protein, His (Fc)-Avi-tagged | +Inquiry |
LIMK1-1176H | Recombinant Human LIMK1 Protein (R329-G638), Tag Free | +Inquiry |
LIMK1-1177H | Recombinant Human LIMK1 Protein (R329-G638), His tagged | +Inquiry |
Limk1-1320M | Recombinant Mouse Limk1 Protein, MYC/DDK-tagged | +Inquiry |
◆ Lysates | ||
LIMK1-4739HCL | Recombinant Human LIMK1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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