Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Active Recombinant Human LIMK1, GST-tagged

Cat.No. : LIMK1-1029H
Product Overview : Recombinant human LIMK1 (285-638) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag.
  • Specification
  • Gene Information
  • Related Products
Description : There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Source : Sf9 insect cells using baculovirus
Species : Human
Tag : GST
Form : Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Bio-activity : 8 nmol/min/mg
Molecular Mass : ~65 kDa
Purity : >95%
Applications : Kinase Assay, Western Blot
Stability : 1 yr at -70°C from date of shipment.
Storage : Store product at –70ºC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration : 0.1ug/ul
Gene Name : LIMK1 LIM domain kinase 1 [ Homo sapiens (human) ]
Official Symbol : LIMK1
Synonyms : LIMK1; LIM domain kinase 1; LIMK; LIM motif-containing protein kinase; LIMK-1; NP_001191355.1; EC 2.7.11.; NP_002305.1
Gene ID : 3984
mRNA Refseq : NM_002314
Protein Refseq : NP_002305
MIM : 601329
UniProt ID : P53667
Chromosome Location : 7q11.23
Pathway : CDC42 signaling events; CXCR4-mediated signaling events; EPHB-mediated forward signaling; Fc gamma R-mediated phagocytosis
Function : ATP binding; heat shock protein binding; protein heterodimerization activity; protein serine/threonine kinase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (0)

Ask a question

Customer Reviews (0)

Write a review

Ask a Question for All LIMK1 Products

Required fields are marked with *

My Review for All LIMK1 Products

Required fields are marked with *

0

Inquiry Basket

cartIcon
logo

FOLLOW US

Terms and Conditions        Privacy Policy

Copyright © 2024 Creative BioMart. All Rights Reserved.

Contact Us

  • /

Stay Updated on the Latest Bioscience Trends