"LIMK1" Related Products

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Recombinant Human LIMK1, GST-tagged

Cat.No.: LIMK1-1029H
Product Overview: Recombinant human LIMK1 (285-638) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag.
Description: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Source: Sf9 insect cells using baculovirus
Species: Human
Tag: GST
Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Bio-activity: 8 nmol/min/mg
Molecular Mass: ~65 kDa
Purity: >95%
Applications: Kinase Assay, Western Blot
Stability: 1 yr at -70°C from date of shipment.
Storage: Store product at –70ºC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration: 0.1ug/ul
Gene Name: LIMK1 LIM domain kinase 1 [ Homo sapiens (human) ]
Official Symbol: LIMK1
Synonyms: LIMK1; LIM domain kinase 1; LIMK; LIM motif-containing protein kinase; LIMK-1; NP_001191355.1; EC 2.7.11.; NP_002305.1
Gene ID: 3984
mRNA Refseq: NM_002314
Protein Refseq: NP_002305
MIM: 601329
UniProt ID: P53667
Chromosome Location: 7q11.23
Pathway: CDC42 signaling events; CXCR4-mediated signaling events; EPHB-mediated forward signaling; Fc gamma R-mediated phagocytosis
Function: ATP binding; heat shock protein binding; protein heterodimerization activity; protein serine/threonine kinase activity

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