| Cat. No. : |
PIP5K1C-1083H |
| Description : |
PIP5K1C or phosphatidylinositol-4-phosphate 5-kinase 1 gamma phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1C is found at synapses and has been found to play roles in endocytosis and cell migration. PIP5K1C directly associates with β-catenin and increases β-catenin activity downstream of growth factor stimulation. PIP5K1C expression and kinase activity enhance β-catenin phosphorylation on residues that promote nuclear importation and transcriptional activity. PIP5K1C deficiency impairs neutrophil recruitment as a result of defect in adhesion. PIP5K1C regulated the adhesion through facilitating RhoA GTPase and integrin activation by chemoattractants. |
| Applications : |
Kinase Assay; Western Blot |
| Molecular Weight : |
120 kDa |
| Expression System : |
Sf9 insect cells using baculovirus |
| Species : |
Human |
| Form : |
Recombinant protein stored in 50mM Tris-HCL, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 0 25% glycerol. |
| Specific Activity : |
220 nmol/min/mg |
| Purity : |
> 80 % |
| Concentration : |
0.1 ug/ul |
| Sequences : |
Full length |
| Storage : |
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
| Pathways : |
3-phosphoinositide biosynthesis; Arf6 trafficking events; Axon guidance; B Cell Receptor Signaling Pathway; E-cadherin signaling in the nascent adherens junction; Endocytosis; Fc gamma R-mediated phagocytosis; Focal Adhesion; Inositol phosphate metabolism; Metabolic pathways; N-cadherin signaling events; Phosphatidylinositol signaling system; Regulation of Actin Cytoskeleton; RhoA signaling pathway; Stabilization and expansion of the E-cadherin adherens junction; superpathway of inositol phosphate compounds |
