Recombinant Human Phosphatase And Tensin Homolog, GST-tagged

• Cat.No.: PTEN-1619H
• Product Overview: Full-length recombinant human PTEN was expressed in E. coli cells using an N-terminal GST tag.

Specification

• Cat. No.: PTEN-1619H
• Description: PTEN is a tumor suppressor that is frequently mutated in a large number of cancers. PTEN has phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity and contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases.
• Applications: Phosphatase Assay; Western Blot
• Molecular Weight: 80 kDa
• Expression System: E. coli
• Species: Human
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
• Specific Activity: 435 nmol/min/mg
• Purity: > 85 %
• Concentration: 0.1 ug/ul
• Sequences: Full length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Endometrial cancer; Focal adhesion; Glioma; Inositol phosphate metabolism; Melanoma; Pathways in cancer; Phosphatidylinositol signaling; Prostate cancer; Small cell lung cancer; Tight junction; p53 signaling pathway; Signaling in Immune system; Signalling by NGF; PIP3 activates AKT signaling

Gene Information

• Gene Name: PTEN phosphatase and tensin homolog [ Homo sapiens ]
• Official Symbol: PTEN
• Synonyms: PTEN; phosphatase and tensin homolog; BZS; DEC; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; MGC11227; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; OTTHUMP00000020032; phosphatase and tensin-like protein; mutated in multiple advanced cancers 1; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; EC 3.1.3.16; EC 3.1.3.48; EC 3.1.3.67; Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog
• Gene ID: 5728
• mRNA Refseq: NM_000314
• Protein Refseq: NP_000305
• MIM: 601728
• UniProt ID: P60484
• Chromosome Location: 10q23
• Function: PDZ domain binding; hydrolase activity; inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity; lipid binding; magnesium ion binding; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; phosphatidylinositol-3-phosphatase activity; platelet-derived growth factor receptor binding; protein binding; protein serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity; enzyme binding