Recombinant Human Ras Homolog Gene Family, Member C, His-tagged

• Cat.No.: RHOC-1628H
• Product Overview: Recombinant full length human RHOC was expressed in E. coli cells using an N-terminal His tag.

Specification

• Cat. No.: RHOC-1628H
• Description: RHOC is member of the Rho family of small GTPases which promote reorganization of the actin cytoskeleton during cell morphogenesis and motility and regulate cell shape, attachment and motility. Active RHOC signals to its downstream effector ROCK which phosphorylates and activates LIM kinase that in turn phosphorylates cofilin inhibiting its actin-depolymerizing activity. Overexpression of RhoC is associated with tumor cell proliferation and metastasis while dominant-negative RHOC inhibits metastasis.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 23 kDa
• Expression System: E. coli
• Species: Human
• Form: Recombinant protein stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.
• Purity: > 75 %
• Concentration: 0.2 ug/ul
• Sequences: Full length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Axon guidance; G alpha (12/13) signalling events; GPCR downstream signaling; Rho GTPase cycle; Sema4D in semaphorin signaling; Sema4D induced cell migration and growth-cone collapse; Semaphorin interactions; Signaling by GPCR; Signaling by Rho GTPases; Signaling events mediated by PRL

Gene Information

• Gene Name: RHOC ras homolog gene family, member C [ Homo sapiens ]
• Official Symbol: RHOC
• Synonyms: RHOC; ras homolog gene family, member C; H9; ARH9; ARHC; RHOH9; MGC1448; MGC61427; rho-related GTP-binding protein RhoC; rhoC GTPase; oncogene RHO H9; rho cDNA clone 9; OTTHUMP00000013675; OTTHUMP00000013676; OTTHUMP00000013802; OTTHUMP00000013803; OTTHUMP00000013804; OTTHUMP00000013805; OTTHUMP00000013806; OTTHUMP00000013807; OTTHUMP00000013809; OTTHUMP00000229713; OTTHUMP00000229714; RAS-related homolog 9; small GTP binding protein RhoC; Rho-related GTP-binding protein RhoC; Rho cDNA clone 9
• Gene ID: 389
• mRNA Refseq: NM_001042678
• Protein Refseq: NP_001036143
• MIM: 165380
• UniProt ID: P08134
• Chromosome Location: 1p13.1
• Function: GTP binding; GTPase activity; protein domain specific binding; nucleotide binding; signal transducer activity