Recombinant Human Sirtuin 4, GST-tagged

• Cat.No.: SIRT4-4879H
• Product Overview: Full-length recombinant human SIRT4 was expressed in E. coli cells using an N-terminal GST tag.

Specification

• Cat. No.: SIRT4-4879H
• Description: SIRT4 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT4 being a member of class IV. SIRT4 lacks deacetylase activity but has ADP-ribosyltransferase activity. Immunoprecipitation analysis shows that SIRT4 interacts with the mitochondrial enzyme glutamate dehydrogenase, and functional analysis show that SIRT4 ADP-ribosylates and inhibits GDH.
• Applications: Western Blot
• Molecular Weight: 62 kDa
• Expression System: E. coli
• Species: Human
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1mM PMSF, 25 % glycerol.
• Purity: > 70 %
• Concentration: 0.2 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Signaling events mediated by HDAC Class I

Gene Information

• Gene Name: SIRT4 sirtuin 4 [ Homo sapiens ]
• Official Symbol: SIRT4
• Synonyms: SIRT4; sirtuin 4; SIR2L4; MGC57437; MGC130046; MGC130047; NAD-dependent ADP-ribosyltransferase sirtuin-4; sir2-like 4; sirtuin type 4; OTTHUMP00000239957; SIR2-like protein 4; sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae); sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 4
• Gene ID: 23409
• mRNA Refseq: NM_012240
• Protein Refseq: NP_036372
• MIM: 604482
• UniProt ID: Q9Y6E7
• Chromosome Location: 12q24.31
• Function: NAD binding; NAD+ ADP-ribosyltransferase activity; NAD-dependent protein deacetylase activity; hydrolase activity; metal ion binding; protein binding; transferase activity; zinc ion binding