Cat. No. :	TLK1-1112H
Product Overview :	Recombinant full-length human TLK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Description :	TLK1 or tousled-like kinase 1 is a nuclear serine/threonine kinase that is potentially involved in the regulation of chromatin assembly. TLK1 is first described in Arabidopsis. TLK1 is a novel target of the DNA damage checkpoint that is rapidly inactivated upon exposure to ionizing radiation (IR) and the inactivation is directly mediated by the S-phase DNA damage checkpoint. The suppression of TLK1 activity after double-strand breaks in the DNA, replication blockade, or low doses of ultraviolet irradiation requires ATM, NBS and CHK1.
Applications :	Kinase Assay; Western Blot
Molecular Weight :	135 kDa
Expression System :	Sf9 insect cells using baculovirus
Species :	Human
Form :	Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
Specific Activity :	6.2 nmol/min/mg
Purity :	> 90 %
Concentration :	0.1 ug/ul
Sequences :	Full-length
Storage :	Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Name :	TLK1 tousled-like kinase 1 [ Homo sapiens ]
Official Symbol :	TLK1
Synonyms :	TLK1; tousled-like kinase 1; KIAA0137; PKU-beta; Serine/threonine-protein kinase tousled-like 1; EC=2.7.11.1; Tousled-like kinase 1; serine threonine protein kinase; SNARE protein kinase SNAK; EC 2.7.11; EC 2.7.11.1; PKU-BETA; OTTHUMP00000205035; OTTHUMP00000205036; OTTHUMP00000205037; OTTHUMP00000205038; OTTHUMP00000205039; OTTHUMP00000225037; OTTHUMP00000163076; OTTHUMP00000163077
Gene ID :	9874
mRNA Refseq :	NM_012290
Protein Refseq :	NP_036422
MIM :	608438
UniProt ID :	Q9UKI8
Chromosome Location :	2q31.1
Function :	ATP binding; protein serine/ threonine kinase activity; nucleotide binding

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