Recombinant Human Tousled-Like Kinase 1, GST-tagged


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Recombinant Human Tousled-Like Kinase 1, GST-tagged

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Cat. No. : TLK1-1112H
Product Overview : Recombinant full-length human TLK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Description : TLK1 or tousled-like kinase 1 is a nuclear serine/threonine kinase that is potentially involved in the regulation of chromatin assembly. TLK1 is first described in Arabidopsis. TLK1 is a novel target of the DNA damage checkpoint that is rapidly inactivated upon exposure to ionizing radiation (IR) and the inactivation is directly mediated by the S-phase DNA damage checkpoint. The suppression of TLK1 activity after double-strand breaks in the DNA, replication blockade, or low doses of ultraviolet irradiation requires ATM, NBS and CHK1.
Applications : Kinase Assay; Western Blot
Molecular Weight : 135 kDa
Expression System : Sf9 insect cells using baculovirus
Species : Human
Form : Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
Specific Activity : 6.2 nmol/min/mg
Purity : > 90 %
Concentration : 0.1 ug/ul
Sequences : Full-lengt h
Storage : Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Gene Name : TLK1 tousled-like kinase 1 [ Homo sapiens ]
Official Symbol : TLK1
Synonyms : TLK1; tousled-like kinase 1; KIAA0137; PKU-beta; Serine/threonine-protein kinase tousled-like 1; EC=; Tousled-like kinase 1; serine threonine protein kinase; SNARE protein kinase SNAK; EC 2.7.11; EC; PKU-BETA; OTTHUMP00000205035; OTTHUMP00000205036; OTTHUMP00000205037; OTTHUMP00000205038; OTTHUMP00000205039; OTTHUMP00000225037; OTTHUMP00000163076; OTTHUMP00000163077
Gene ID : 9874
mRNA Refseq : NM_012290
Protein Refseq : NP_036422
MIM : 608438
UniProt ID : Q9UKI8
Chromosome Location : 2q31.1
Function : ATP binding; protein serine/ threonine kinase activity; nucleotide binding

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