| Cat. No. : |
TLK1-1112H |
| Product Overview : |
Recombinant full-length human TLK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. |
| Description : |
TLK1 or tousled-like kinase 1 is a nuclear serine/threonine kinase that is potentially involved in the regulation of chromatin assembly. TLK1 is first described in Arabidopsis. TLK1 is a novel target of the DNA damage checkpoint that is rapidly inactivated upon exposure to ionizing radiation (IR) and the inactivation is directly mediated by the S-phase DNA damage checkpoint. The suppression of TLK1 activity after double-strand breaks in the DNA, replication blockade, or low doses of ultraviolet irradiation requires ATM, NBS and CHK1. |
| Applications : |
Kinase Assay; Western Blot |
| Molecular Weight : |
135 kDa |
| Expression System : |
Sf9 insect cells using baculovirus |
| Species : |
Human |
| Form : |
Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol. |
| Specific Activity : |
6.2 nmol/min/mg |
| Purity : |
> 90 % |
| Concentration : |
0.1 ug/ul |
| Sequences : |
Full-lengt h |
| Storage : |
Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
