||Recombinant human CCND2 protein, fused to His-tag at N-terminus, was expressed in E.coli.
||CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of this protein was observed in ovarian and testicular tumors.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 1M Urea.
||35.6 kDa (313aa)
||> 85% by SDS-PAGE
||1 mg/ml (determined by Bradford)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MGSHMELLCH EVDPVRRAVR DRNLLRDDRV LQNLLTIEER YLPQCSYFKC VQKDIQPYMR RMVATWMLEV CEEQKCEEEV FPLAMNYLDR FLAGVPTPKS HLQLLGAVCM FLASKLKETS PLTAEKLCIY TDNSIKPQEL LEWELVVLGK LKWNLAAVTP HDFIEHILRK LPQQREKLSL IRKHAQTFIA LCATDFKFAM YPPSMIATGS VGAAICGLQQ DEEVSSLTCD ALTELLAKIT NTDVDCLKAC QEQIEAVLLN SLQQYRQDQR DGSKSEDELD QASTPTDVRD IDL
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||B Cell Receptor Signaling Pathway; Cell Cycle, Mitotic; Cyclin D associated events in G1; DNA damage response (only ATM dependent); Focal Adhesion; G1 Phase; G1 to S cell cycle control; IL-7 Signaling Pathway; IL2 signaling events mediated by STAT5; Jak-STAT signaling pathway; Measles; Mitotic G1-G1/S phases; Ovarian Infertility Genes; Regulation of Wnt-mediated beta catenin signaling and target gene transcription; Validated targets of C-MYC transcriptional activation; Wnt Signaling Pathway and Pluripotency; p53 signaling pathway