||Recombinant human RAB2B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||RAB2B belongs to the small GTPase superfamily. Members of the Rab protein family are nontransforming monomeric GTP-binding proteins of the Ras superfamily that contain 4 highly conserved regions involved in GTP binding and hydrolysis. Rab proteins are prenylated, membrane-bound proteins involved in vesicular fusion and trafficking.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing10% glycerol, 1mM DTT, 0.15M NaCl.
||26.8 kDa (240aa), confirmed by MALDI-TOF.
||>95% by SDS-PAGE
||1 mg/ml (determined by Bradford)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MGSHMTYAYL FKYIIIGDTG VGKSCLLLQF TDKRFQPVHD LTIGVEFGAR MVNIDGKQIK LQIWDTAGQE SFRSITRSYY RGAAGALLVY DITRRETFNH LTSWLEDARQ HSSSNMVIML IGNKSDLESR RDVKREEGEA FAREHGLIFM ETSAKTACNV EEAFINTAKE IYRKIQQGLF DVHNEANGIK IGPQQSISTS VGPSASQRNS RDIGSNSGCC
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.