"HK1" Related Products

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Recombinant Human HK1 Protein, His-tagged

Cat.No. : HK1-1141H
Product Overview : Recombinant Human HK1 Protein (1-917 aa) is produced by E. coli expression system. This protein is fused with a His-tag.
Description : Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this hexokinase1 have been associated with hemolytic anemia due to hexokinase deficiency. Recombinant human Hexokinase1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Source : E. coli
Species : Human
Tag : His
Form : Liquid. In 20mM Tris-HCl pH8.0, 10% glycerol
Bio-activity : Specific activity is > 8,000 pmol/min/ug. One unit will convert 1 pmoles of D-Glucose to D-Glucose-6-phosphate per minute at pH 7.5 at 37 centigrade.
Molecular Mass : 104.6 kDa
Protein length : 1-917 aa
AA Sequence : MGSSHHHHHH SSGLVPRGSH MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDETLIDIM TRFRKEMKNG LSRDFNPTAT VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE
KNQNVHMESE VYDTPENIVH GSGSQLFDHV AECLGDFMEK RKIKDKKLPV GFTFSFPCQQ SKIDEAILIT WTKRFKASGV EGADVVKLLN KAIKKRGDYD ANIVAVVNDT VGTMMTCGYD
DQHCEVGLII GTGTNACYME ELRHIDLVEG DEGRMCINTE WGAFGDDGSL EDIRTEFDRE IDRGSLNPGK QLFEKMVSGM YLGELVRLIL VKMAKEGLLF EGRITPELLT RGKFNTSDVS
AIEKNKEGLH NAKEILTRLG VEPSDDDCVS VQHVCTIVSF RSANLVAATL GAILNRLRDN KGTPRLRTTV GVDGSLYKTH PQYSRRFHKT LRRLVPDSDV RFLLSESGSG KGAAMVTAVA
YRLAEQHRQI EETLAHFHLT KDMLLEVKKR MRAEMELGLR KQTHNNAVVK MLPSFVRRTP DGTENGDFLA LDLGGTNFRV LLVKIRSGKK RTVEMHNKIY AIPIEIMQGT GEELFDHIVS
CISDFLDYMG IKGPRMPLGF TFSFPCQQTS LDAGILITWT KGFKATDCVG HDVVTLLRDA IKRREEFDLD VVAVVNDTVG TMMTCAYEEP TCEVGLIVGT GSNACYMEEM KNVEMVEGDQ
GQMCINMEWG AFGDNGCLDD IRTHYDRLVD EYSLNAGKQR YEKMISGMYL GEIVRNILID FTKKGFLFRG QISETLKTRG IFETKFLSQI ESDRLALLQV RAILQQLGLN STCDDSILVK
TVCGVVSRRA AQLCGAGMAA VVDKIRENRG LDRLNVTVGV DGTLYKLHPH FSRIMHQTVK ELSPKCNVSF LLSEDGSGKG AALITAVGVR LRTEASS
Purity : > 90% by SDS - PAGE
Applications : Enzyme Activity; SDS-PAGE
Storage : Can be stored at +2 centigrade to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/ml (determined by Bradford assay)
Figure :

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Gene Name : HK1 hexokinase 1 [ Homo sapiens ]
Official Symbol : HK1
Synonyms : HK1; hexokinase 1; hexokinase-1; HK I; glycolytic enzyme; hexokinase type I; HKI; HXK1; HK1-ta; HK1-tb; HK1-tc;
Gene ID : 3098
mRNA Refseq : NM_000188
Protein Refseq : NP_000179
MIM : 142600
UniProt ID : P19367

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