Recombinant Human Cyclin-Dependent Kinase 7, His-tagged

• Cat.No.: CDK7-1151H
• Product Overview: A DNA sequence encoding the human CDK7 (P50613) (Ala 2 - Phe 346) was fused with a polyhistidine tag at the N-terminus.

Specification

• Cat. No.: CDK7-1151H
• Description: CDK7, also known as 39 kDa protein kinase, CAK1, CDK-activating kinase, Cell division protein kinase 7, TFIIH basal transcription factor complex kinase subunit and CDK7, is a nucleus protein which Belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily.
• Source: Human
• Host: Human Cells
• Form: Lyophilized from sterile PBS, pH7.4. Normally 5% - 8% trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
• Purity: > 85% as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70°C.
• Predicted N terminal: Met
• Molecular Mass: The recombinant heterotrimer of human CDK7/CCNH/MNAT1 comprises 1032 (364 + 341 + 327) amino acids and has a calculated molecular mass of 118.8 (41.2 + 39.7 + 37.9) kDa. The apparent molecular mass of rh CDK7/CCNH/MNAT1 heterotrimer is approximately 25, 38 & 44 kDa respectively in SDS-PAGE under reducing conditions.
• Application: SDS-PAGE
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Storage: Store it under sterile conditions at -70°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Pathways: Androgen Receptor Signaling Pathway; B Cell Receptor Signaling Pathway; DNA Repair; Eukaryotic Transcription Initiation; HIV Infection; Late Phase of HIV Life Cycle; MicroRNAs in cardiomyocyte hypertrophy; Nucleotide Excision Repair; S Phase; Transcription

Gene Information

• Gene Name: CDK7 cyclin-dependent kinase 7 [ Homo sapiens ]
• Official Symbol: CDK7
• Synonyms: CDK7; cyclin-dependent kinase 7; CAK1; MO15; STK1; CDKN7; p39MO15; CAK; p39 Mo15; 39 KDa protein kinase; Cdk-activating kinase; kinase subunit of CAK; serine/threonine kinase stk1; cell division protein kinase 7; serine/threonine protein kinase 1; serine/ threonine protein kinase MO15; cyclin-dependent kinase 7 (homolog of Xenopus MO15 Cdk-activating kinase); TFIIH basal transcription factor complex kinase subunit; cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase)
• Gene ID: 1022
• mRNA Refseq: NM_001799
• Protein Refseq: NP_001790
• MIM: 601955
• UniProt ID: P50613
• Chromosome Location: 5q12.1
• Function: ATP binding; DNA-dependent ATPase activity; RNA polymerase II carboxy-terminal domain kinase activity; androgen receptor binding; kinase activity; nucleotide binding; protein C-terminus binding; protein complex binding; protein kinase activity; transcription coactivator activity