"Il3" Related Products


Recombinant Mouse Interleukin-3

Cat. No.: Il3-09M
Product Overview: Recombinant mouse interleukin-3 produced in E. coli is a single, non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 15,100 Dalton. The IL-3 is purified by proprietary chromatographic techniques.
Description: Interleukin-3 is a pleiotropic cytokine produced primarily by activated T cells.IL-3 is thought to function via specific cell surface receptors to stimulate the proliferation, differentiation and survival of haematopoietic cell lines. IL-3 has also been shown to affect the functional activity of a variety of other cell types including mast cells, eosinophils, megakaryocytes and basophils.
Purity: >95% as determined by SDS-PAGE and RP-HPLC.
Formulation: Lyophilized from concentrated (1mg/ml) solution in in water containing no additives.
Specific Activity: The recombinant mouse IL-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine MC-9 cells is less than 1ng/ml, corresponding to a Specific Activity of >1 x 106IU/mg.
Endotoxin: Less than 1 ng/ug(IEU/μg) of mouse IL-3 as determined by LAL method.
Reconstitution: It is recommended to reconstitute the lyophilized mouse IL-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage: Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-3 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles.
Gene Name: interleukin 3(Mus musculus)
Synonyms: interleukin 3; BPA; PSF; HCGF; Il-3; MCGF; Csfmu; OTTMUSP00000005831; Il3
Gene ID: 16187
mRNA Refseq: NM_010556
Protein Refseq: NP_034686
MIM: 147740
UniProt ID: P01586
Chromosome Location: 11 B1; 11 28.5 cM
Pathway: Apoptosis; Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Hematopoietic cell lineage; Jak-STAT signaling pathway .
Function: Cytokine activity; growth factor activity; interleukin-3 receptor binding.

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