||Recombinant mouse interleukin-3 produced in E. coli is a single, non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 15,100 Dalton. The IL-3 is purified by proprietary chromatographic techniques.
||Interleukin-3 is a pleiotropic cytokine produced primarily by activated T cells.IL-3 is thought to function via specific cell surface receptors to stimulate the proliferation, differentiation and survival of haematopoietic cell lines. IL-3 has also been shown to affect the functional activity of a variety of other cell types including mast cells, eosinophils, megakaryocytes and basophils.
||>95% as determined by SDS-PAGE and RP-HPLC.
||Lyophilized from concentrated (1mg/ml) solution in in water containing no additives.
||The recombinant mouse IL-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine MC-9 cells is less than 1ng/ml, corresponding to a Specific Activity of >1 x 106IU/mg.
||Less than 1 ng/ug(IEU/μg) of mouse IL-3 as determined by LAL method.
||It is recommended to reconstitute the lyophilized mouse IL-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
||Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-3 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles.