||Recombinant Human IL-15 produced in E. coli is a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 12774 Dalton.
||Interleukin-15 (IL-15) is a cytokine that possesses a variety of biological functions, including stimulation and maintenance of cellular immune responses. IL-15 stimulates the proliferation of T-lymphocytes, which requires interaction of IL-15 with components of IL-2R, including IL-2R and probably IL-2R gamma, but not IL-2R.
||>98% as determined by RP-HPLC, FPLC and SDS-PAGE .
||The protein was lyophilized from a concentrated (1mg/ml) solution with 5mM Tris pH 8.0.
||The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be < 0.5 ng/ml, corresponding to a Specific Activity of 2.0 x 106IU/mg.
||Less than 0.1 ng/µg (IEU/µg) of rHuIL-15.
||It is recommended to reconstitute the lyophilized rHuIL-15 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
||Lyophilized rHuIL-15 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-15 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.