| Description : |
Protein disulfide isomerases (PDIs) constitute a family of structurally related enzymes which catalyze disulfide bonds formation, reduction, or isomerization of newly synthesized proteins in the lumen of the endoplasmic reticulum (ER). They act also as chaperones, and are, therefore, part of a quality-control system for the correct folding of the proteins in the same subcellular compartment. PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro.Recombinant Human Protein Disulfide Isomerase is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. Recombinant PDI has been found to have moderate effects (25-fold) on the rate of oxidative folding of proteins in vitro. |
| Source : |
E.coli |
| Species : |
Human |
| Form : |
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.0. |
| Bio-activity : |
Isomerase Activity is 0.5 µmol active RNase A min-1 µmol PDI-1, according to the re-activation of reduced and denatured RNase A. |
| Molecular Mass : |
Approximately 56.6 kDa, a single non-glycosylated polypeptide chain containing 502 amino acids. (MRGSGSHHHHHH-PDI). |
| Protein length : |
502 |
| AA Sequence : |
MRGSGSHHHHHHAPEEEDHVLVLRK SNFAEALAAHKYLLVEFYAPWCGHC KALAPEYAKAAGKLKAEGSEIRLAK VDATEESDLAQQYGVRGYPTIKFFR NGDTASPKEYTAGREADDIVNWLKK RTGPAATTLPDGAAAESLVESSEVA VIGFFKDVESDSAKQFLQAAEAIDD IPFGITSNSDVFSKYQLDKDGVVLF KKFDEGRNNFEGEVTKENLLDFIKH NQLPLVIEFTEQTAPKIFGGEIKTH ILLFLPKSVSDYDGKLSNFKTAAES FKGKILFIFIDSDHTDNQRILEFFG LKKEECPAVRLITLEEEMTKYKPES EELTAERITEFCHRFLEGKIKPHLM SQELPEDWDKQPVKVLVGKNFEDVA FDEKKNVFVEFYAPWCGHCKQLAPI WDKLGETYKDHENIVIAKMDSTANE VEAVKVHSFPTLKFFPASADRTVID YNGERTLDGFKKFLESGGQDGAGDD DDLEDLEEAEEPDMEEDDDQKAVKD EL |
| Endotoxin : |
Less than 1 EU/µg of rHuPDI as determined by LAL method. |
| Purity : |
>95% by SDS-PAGE and HPLC analysis. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
