"Hirudin" Related Products

Recombinant Hirudin

Cat. No.: Hirudin-02
Product Overview: Recombinant Hirudin produced inPichia. Pastoriscontains 65 amino acid residues and having a molecular mass of 6979.5 Da.
Description: Hirudin is a potent thrombin inhibitor originally derived from the medicinal leech Unlike heparin, hirudin act directly on thrombin, rather than through other clotting factors. They have a high binding affinity and specificity for thrombin. The mechanism of hirudin-thrombin binding appears to be unique. Recombinant hirudin variant is derived from yeast cell, the polypeptide containing 65 amino acid residues has a molecular weight of 6979.5 Da, which is identical to natural hirudin except for substitution of leucine for isoleucine at the N-terminal end of the molecule and the absence of a sulfate group on the tyrosine at position 63.
Purity: >96% by SDS-PAGE and HPLC analyses.
CAS Number: 8001-27-2
Formulation: Lyophilized from a 0.2μm filtered solution of 20mM PBS, pH 7.0, containing 2% mannitol.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Production: Yeast expression of a DNA sequence encoding the mature hirudin variant (Leu1,Thr2-63-desulfo hirudin).
Specific Activity: rHirudin is fully biologically active when compared to standard. Its specific activity is ≥1×104ATU/mg.
Endotoxin: Less than 10EU/mg of rhirudin as determined by LAL method.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20oC. Further dilutions should be made in appropriate buffered solutions.
Storage: This lyophilized preparation is stable at 2-8oC, but should be kept at -20oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20oC. Avoid repeated freeze/thaw cycles.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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