Active Recombinant Human PLAT protein(Ile311-Pro562), hFc-tagged

• Cat.No.: PLAT-72H
• Product Overview: Recombinant Human tPA (NP_000921.1)(Ile 311-Pro 562) was expressed in HEK293, obtained after cleavage of the N-terminal Human IgG1 Fc region from the purified chimera.

Specification

• Source: HEK293
• Species: Human
• Tag: N-hFc
• Protein length: Ile311-Pro562
• Form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a peptide substRate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin. The specific activity is > 300 pmols/min/μg.
• Molecular Mass: The recombinant human tPA β chain consists of 252 amino acids and has a predicted molecular mass of 28 kDa. As a result of glycosylation, the apparent molecular mass of rh tPAβ is approximately 38 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PLAT plasminogen activator, tissue [ Homo sapiens ]
• Official Symbol: PLAT
• Synonyms: PLAT; plasminogen activator, tissue; tissue-type plasminogen activator; alteplase; reteplase; t-plasminogen activator; plasminogen activator, tissue type; tissue plasminogen activator (t-PA); TPA; T-PA; DKFZp686I03148
• Gene ID: 5327
• mRNA Refseq: NM_000930
• Protein Refseq: NP_000921
• MIM: 173370
• UniProt ID: P00750