Recombinant Human EPO protein,Fc-tagged

• Cat.No.: EPO-97H
• Product Overview: Recombinant Human EPO alpha protein was expressed in CHO cells with Fc tag.

Specification

• Species: Human
• Source: CHO
• Tag: Fc
• Description: Erythropoietin (EPO), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The production of EPO by kidney cells is increased in response to hypoxia or anemia. Recombinant EPO has been approved for the treatment of anemia associated with chronic renal failure as well as for anemia of AZT treated AIDS patients.
• Form: Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
• Bio-activity: Fully biologically active when compared to standard. The Specific Activity was measured by the stimulation of reticulocyte production in normocyth-aemic mice and is no less than 5.0 × 10⁵ IU/mg.
• Molecular Mass: Recombinant Human EPO/Fc produced in CHO is a dimeric, glycosylated, polypeptide chain consisting of two mature human EPO molecules linked to the Fc portion of human IgG1. The Fc component contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 140 kDa in non-reducing SDS-PAGE.
• Endotoxin: Less than 1 EU/μg of rHuEPO-Fc a as determined by LAL method.
• Purity: >98% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: EPO
• Official Symbol: EPO
• Synonyms: EPO; erythropoietin; EP; epoetin; MVCD2; MGC138142
• Gene ID: 2056
• mRNA Refseq: NM_000799
• Protein Refseq: NP_000790
• MIM: 133170
• UniProt ID: P01588

Citation

Dual Delivery of EPO and BMP2 from a Novel Modular Poly-?-Caprolactone Construct to Increase the Bone Formation in Prefabricated Bone Flaps

Journal: Tissue Engineering Part C: Methods    Data: 2015/9/1

Authors: Janki Jayesh Patel, Jane E. Modes, Scott J. Hollister

Article Snippet:After manufacturing, the scaffolds were air blasted, sonicated in 70% ethanol (EtOH), sterilized in 0.22μm filtered 70% EtOH, and airdried under sterile conditions.After manufacturing, the scaffolds were air blasted, sonicated in 70% ethanol (EtOH), sterilized in 0.22μm filtered 70% EtOH, and airdried under sterile conditions.. EPO Binding to PCL Scaffolds 100μg EPO (Creative BioMart, Shirley, NY) was reconstituted in 1ml sterile Dulbecco’s phosphate buffered saline (DPBS) to result in 12,000 IU/ml (0.1mg/ml).. The stock solution was further diluted to the desired concentrations in DPBS.The stock solution was further diluted to the desired concentrations in DPBS.

Oxalic acid supported Si-18F-radiofluorination: one-step radiosynthesis of N-succinimidyl 3-(di-tert-butyl[18F]fluorosilyl)benzoate ([18F]SiFB) for protein labeling.

Journal: Bioconjugate chemistry    PubMed ID: 22148255    Data: 2012/6/15

Authors: Alexey P Kostikov, Joshua Chin, Ralf Schirrmacher

Article Snippet:All other commercially available chemicals, such as Kryptofix 2.2.2, acetonitrile, potassium oxalate, oxalic acid, tetrapropylammonium hydroxide, and N,N,N′,N′tetramethyl-O-(N-succinimidyl)uronium tetrafluoroborate, and RSA, apo-transferrin and IgG, were purchased at the highest available purity from Sigma-Aldrich.All other commercially available chemicals, such as Kryptofix 2.2.2, acetonitrile, potassium oxalate, oxalic acid, tetrapropylammonium hydroxide, and N,N,N′,N′tetramethyl-O-(N-succinimidyl)uronium tetrafluoroborate, and RSA, apo-transferrin and IgG, were purchased at the highest available purity from Sigma-Aldrich.. Recombinant erythropoitin (EPO) was purchased from Creative BioMart (US).. The beta cell SCA was provided by Dr. S. Schneider (Innere Medizin II, Diabetologie und Endokrinologie St. Vinzenz-Hospital, Universitat? zu Ko?ln).The beta cell SCA was provided by Dr. S. Schneider (Innere Medizin II, Diabetologie und Endokrinologie St. Vinzenz-Hospital, Universitat? zu Ko?ln).