| Cat. No. : |
LEP-08C |
| Description : |
Leptin (Greek leptos meaning thin) is a 16 kDa protein hormone that plays a key role in regulating energy intake and energy expenditure, including appetite and metabolism. It is one of the most important adipose derived hormones. |
| Source : |
Escherichia Coli. |
| Amino Acid Sequence : |
The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Ile-Asp-Val-Asn-Ile |
| Physical Appearance : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Purity : |
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Formulation : |
The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045 mM NaHCO3. |
| Solubility : |
The lyophilized Leptin Binding Domain is very soluble in water and most aqueous buffers below and above the isoelectric point. |
| Biological Activity : |
Biological Activity is evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio. |
| Protein content : |
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.45 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Leptin Binding Domain as a Reference Standard. |
| Storage : |
Lyophilized Leptin Binding Domain although stable at room temperature, should be stored desiccated below 0℃. Reconstituted Leptin is best stored refrigerated at 4℃.Please prevent freeze-thaw cycles. |
| Gene Name : |
LEP leptin [ Gallus gallus ] |
| Synonyms : |
OB Protein; Obesity Protein; OBS; Obesity factor; LEP; Lep; leptin; ob; obese; obesity factor; leptin (murine obesity homolog); leptin (obesity homolog, mouse); FLJ94114; Obese protein |
| Gene ID : |
373955 |
