Recombinant Ovine Prolactin, 199aa
Cat.No. : | PRL-59O |
Product Overview : | Recombinant Ovine Prolactin produced inE.Coliis a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 23 kDa. The Prolactin n is purified by proprietary chromatographic techniques. |
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- Gene Information
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Cat. No. : | PRL-59O |
Description : | Prolactin is a neuroendocrine hormone synthesized primarily by the pituitary gland but also a variety of other cell types including the placenta, brain and uterus. Its primary function is to promote and maintain lactation but has also been shown to have a role in breast cancer development, regulation of reproductive function and immunoregulation. |
Source : | Escherichia Coli. |
Physical Appearance : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity : | Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : | The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045 mM NaHCO3. |
Solubility : | It is recommended to reconstitute the lyophilized Prolactin in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
Amino Acid Sequence : | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val-Cys-Pro. |
Biological Activity : | Is fully biologically active as evidenced by inducing proliferation of Nb2 cells. |
Protein Content : | Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Prolactin as a Reference Standard. |
Storage : | Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prl should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Gene Name : | PRL prolactin [ Ovis aries ] |
Synonyms : | Prolactin; PRL;prolactin |
Gene ID : | 443317 |
mRNA Refseq : | NM_001009306 |
Protein Refseq : | NP_001009306 |
UniProt ID : | P01240 |
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◆ Native Protein | ||
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◆ Lysates | ||
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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