||Recombinant Human MECP2 (78-162 a.a.) fused with GST-tag at N-terminal, was expressed in E. coli expression system..
||DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
||Useful for the study of DNA binding, screening inhibitors, and selectivity profiling.
||45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.2 mM KCl, 18 mM glutathione, 10% glycerol and 3 mM DTT.
||>6 months at –80°C