| Description : |
The CCAAT-box binding protein 1 (CTF1, also called NF-I or NF-1) is a proline-rich transcription activator. It selectively activates transcription of genes that contain GCCAAT consensus sequence in promoter region, including human Ha-Ras, alpha-globin, hsp70 and many other cellular and viral genes (1, 3-5). Cloning and functional characterization of the protein revealed that CTF1 contains an N-terminal DNA binding domain and a C-terminal proline-rich activation domain. CTF1 was shown to interact with the general transcription factor IIB (TFIIB) and facilitate the recruitment of TFIIB to TBP-DNA complexes, with the forkhead thyroid transcription factor TTF2 to regulate hormone–dependent transcription of thyroperoxidase gene, as well as other receptor proteins to synergize hormone receptor-responsive activation. |
| Source : |
Sf21 Cells |
| Species : |
Human |
| Tag : |
His |
| Form : |
20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA |
| Bio-activity : |
1 unit equals 1 ng purified protein. 30-100 units (ng) are required for a reconstituted transcription assay and 5-20 units (ng) are required for a gel mobility shift assay in a 20μl reaction. |
| AA Sequence : |
MDEFHPFIEA LLPHVRAFAY TWFNLQARKR KYFKKHEKRM SKDEERAVKD ELLGEKPEVK QKWASRLLAK LRKDIRPECR EDFVLSITGK KAPGCVLSNP DQKGKMRRID CLRQADKVWR LDLVMVILFK GIPLESTDGE RLVKAAQCGH PVLCVQPHHI GVAVKELDLY LAYFVRERDA EQSGSPRTGM GSDQEDSKPI TLDTTDFQES FVTSGVFSVT ELIQVSRTPV VTGTGPNFSL GELQGHLAYD LNPASTGLRR TLPSTSSSGS KRHKSGSMEE DVDTSPGGDY YTSPSSPTSS SRNWTEDMEG GISSPVKKTE MDKSPFNSPS PQDSPRLSSF TQHHRPVIAV HSGIARSPHP SSALHFPTTS ILPQTASTYF PHTAIRYPPH LNPQDPLKDL VSLACDPASQ QPGPLNGSGQ LKMPSHCLSA QMLAPPPPGL PRLALPPATK PATTSEGGAT SPTSPSYSPP DTSPANRSFV GLGPRDPAGI YQAQSWYLG |
| Purity : |
>95% as determined by SDS-PAGE |
| Storage : |
-80 °C |
| Concentration : |
500 μg/ml |
| Shipping : |
Dry Ice |
