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Recombinant Human GTF2A1/GTF2A2, His-tagged

Cat.No.: GTF2A1-8476H
Product Overview: Recombinant Human GTF2A1/GTF2A2, fused with His tag, was expressed in E. coli.
Description: The transcription factor IIA (TFIIA) has been shown to bind to the TBP-DNA complex and to increase the affinity of TBP for the TATA element. Human TFIIA consists of three subunits of 35 kDa (alpha subunit), 19 kDa (beta subunit) and 12 kDa (gamma subunit). The alpha and beta subunits are derived from the product, p55, of a single gene by an unknown mechanism. However, recombinant p55, in combination with a 12 kDa subunit (gamma subunit), retains native TFIIA activity .
Source: E. coli
Species: Human
Tag: His
Form: 20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
Bio-activity: 1 unit equals 1 ng of purified protein. 1 unit is sufficient for a gel mobility shift assay in a 20μl reaction; 20 units are sufficient for an in vitro transcription assay and 100 units are sufficient for protein-protein interaction assays.
Purity: >95% as determined by SDS-PAGE
Storage: -80 °C
Concentration: 150 μg/ml
Shipping: Dry Ice
Gene Name: GTF2A1 general transcription factor IIA, 1, 19/37kDa [ Homo sapiens ]
Official Symbol: GTF2A1
Synonyms: GTF2A1; general transcription factor IIA, 1, 19/37kDa; TF2A1; TFIIA; TFIIAL; TFIIA-42; transcription initiation factor IIA subunit 1; TFIIA alpha, p55, isoform 1; general transcription factor IIA subunit 1; transcription initiation factor TFIIA 42 kDa subunit; glucose regulated protein, 58kD pseudogene
Gene ID: 2957
mRNA Refseq: NM_015859
Protein Refseq: NP_056943
MIM: 600520
UniProt ID: P52655
Chromosome Location: 14q31.1
Pathway: Basal transcription factors, organism-specific biosystem; Disease; Viral carcinogenesis
Function: DNA binding; protein binding; TBP-class protein binding

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