Active Recombinant Human SRSF2, His-tagged

• Cat.No.: SRSF2-8495H
• Product Overview: Recombinant Human SRSF2, fused with His tag, was expressed in Sf21 Cells.

Specification

• Description: SC35, a member of the SR protein family, is an essential pre-mRNA splicing factor and can function as an essential splicing factor as well as an alternative splicing factor. Phosphorylation of serine residues located within the SR domain directly regulates SC35 activity and compartmentalization of other SR splicing factors. In addition to interacting with RNA and other splicing factors, SC35 has been shown to interact either directly or indirectly with the C-terminal domain (CTD) of the largest subunit of RNA polymerase II, thereby suggesting a potential role of SC35 in coordinating transcription and pre-mRNA splicing.
• Source: Sf21 Cells
• Species: Human
• Tag: His
• Form: 20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
• Bio-activity: 1 unit equals 1 ng of purified protein. 1-5 units are the amount sufficient for a gel mobility shift assay in a 20 μl reaction, 100 units are sufficient for a protein-protein interaction assay and 100-500 units are required for a splicing assay in a 20 μl reaction.
• AA Sequence: GRPPPDVEGM TSLKVDNLTY RTSPDTLRRV FEKYGRVGDV YIPRDRYTKE SRGFAFVRFH DKRDAEDAMD AMDGAVLDGR ELRVQMARYG RPPDSHHSRR GPPPRRYGGG GYGRRSRSPR RRRRSRSRSR SRSRSRSRSR YSRSKSRSRT RSRSRSTSKS RSARRSKSKS SSVSRSRSRS RSRSRSRSPP PVSKRESKSR SRSKSPPKSP EEEGAVSS
• Purity: >95% as determined by SDS-PAGE
• Storage: -80 °C
• Concentration: 650 μg/ml
• Shipping: Dry Ice
• Publication:
  Mutations Causing X-Linked Amelogenesis Imperfecta Alter miRNA Formation from Amelogenin Exon4 (2023)

Gene Information

• Gene Name: SRSF2 serine/arginine-rich splicing factor 2 [ Homo sapiens ]
• Official Symbol: SRSF2
• Synonyms: SRSF2; serine/arginine-rich splicing factor 2; SFRS2, splicing factor, arginine/serine rich 2; PR264; SC 35; SC35; SFRS2A; SR splicing factor 2; splicing factor SC35; splicing component, 35 kDa; splicing factor, arginine/serine-rich 2; SC-35; SFRS2; SRp30b
• Gene ID: 6427
• mRNA Refseq: NM_003016
• Protein Refseq: NP_003007
• MIM: 600813
• UniProt ID: Q01130
• Chromosome Location: 17q25.2
• Pathway: Cleavage of Growing Transcript in the Termination Region, organism-specific biosystem; Gene Expression, organism-specific biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; RNA Polymerase II Transcription, organism-specific biosystem; RNA Polymerase II Transcription Termination, organism-specific biosystem
• Function: RNA binding; nucleic acid binding; nucleotide binding; protein binding; transcription corepressor activity