| Cat.No. : |
CD3E-8541H |
| Product Overview : |
Recombinant Human CD3E (Asp23-Asp126) protein was fused to human IgG1 Fc/His-tag at C-terminus and expressed in human 293 cells (HEK293). |
| Description : |
CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
His/human IgG1 Fc |
| Predicted N Terminal : |
Asp23 |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. |
| Molecular Mass : |
The protein has a calculated MW of 39.2 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein length : |
Asp23-Asp126 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
| SDS-PAGE of CD3E-8541H : |
Human CD3 epsilon, Fc,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|
| Bioactivity-ELISA of CD3E-8541H : |
Immobilized Anti Human CD3 MAb, mouse IgG1 at 2 μg/mL (100 μL/well) can bind Human CD3 epsilon, Fc,His Tag with a linear range of 0.1-3 ng/mL (Routinely tested).
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.