Active Recombinant Mouse Hgf protein(Met1-Leu728), His-tagged

• Cat.No.: Hgf-8664M
• Product Overview: Recombinant Mouse HGF (Q08048-1) (Met1-Leu728) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Leu728
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell prolifeRation. The ED50 for this effect is typically 1-8 ng/mL.
• Molecular Mass: The recombinant mouse HGF comprises 707 amino acids and has a predicted molecular mass of 80.8 kDa. The apparent molecular mass of the protein is approximately 92 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 70 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Hgf hepatocyte growth factor [ Mus musculus ]
• Official Symbol: Hgf
• Synonyms: HGF; hepatocyte growth factor; SF; scatter factor; hepatopoeitin-A; hepatopoietin-A; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik
• Gene ID: 15234
• mRNA Refseq: NM_010427
• Protein Refseq: NP_034557