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Active Recombinant Rat Cd47, His tagged

Cat.No. : Cd47-8767R
Product Overview : Recombinant Rat Cd47 (P97829-1) (Met1-Lys140), fused with a polyhistidine tag at the C-terminus, was produced in Human Cell.
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Source : HEK293
Species : Rat
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4
Bio-activity : Measured by its binding ability in a functional ELISA.
Immobilized Rat CD47-His at 10 μg/ml (100 μl/well) can bind mouse SIRPA-Fc, The EC50 of mouse SIRPA-Fc is 0.6-1.4 μg/ml.
Molecular Mass : The recombinant rat CD47 comprises 133 amino acids and predicts a molecular mass of 15.2 kDa. The apparent molecular mass of the recombinant protein is approximately 27-35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Protein Length : Met1-Lys140
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 90 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Cd47 integrin-associated protein [ Rattus norvegicus ]
Official Symbol : Cd47
Synonyms : CD47; integrin-associated protein;
Gene ID : 113934

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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