Recombinant Rat Cd5, Fc-tagged

• Cat.No.: Cd5-8797R
• Product Overview: A DNA sequence encoding the rat FZD4 (Q9QZH0) (Met1-Glu181) was expressed with the Fc region of human IgG1 at the C-terminus.

Specification

• Description: FZD4, also known as CD344, belongs to the the frizzle family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. FZD4 is the receptor for Wnt proteins. In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin, promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. FZD4 plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin.
• Source: Human Cells
• Species: Rat
• Tag: Fc
• Predicted N Terminal: Phe 38
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant rat FZD4 is a disulfide-linked homodimer. The reduced monomer comprises 385 amino acids and has a predicted molecular mass of 43.2 kDa. The apparent molecular mass of the protein is approximately 113, 58 and 36 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: 1.0 eu/μg of the protein as determined by the lal
• Purity: >(13.3+78.9+5.2 )% as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Cd5 Cd5 molecule [ Rattus norvegicus ]
• Official Symbol: Cd5
• Synonyms: CD5; Cd5 molecule; T-cell surface glycoprotein CD5; CD5 antigen; Lymphocyte antigen CD5; MGC114334
• Gene ID: 54236
• mRNA Refseq: NM_019295
• Protein Refseq: NP_062168
• Pathway: B Cell Receptor Signaling Pathway, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; T Cell Receptor Signaling Pathway, organism-specific biosystem
• Function: glycoprotein binding; scavenger receptor activity