Active Recombinant Human IL3, His-tagged

• Cat.No.: IL3-8862H
• Product Overview: Recombinant Human IL3 was produced in the endosperm tissue of barley grain (Hordeum vulgare).

Specification

• Description: IL-3 (Interleukin-3, Hematopoietic growth factor, Mast cell growth factor), a pleiotropic growth factor, is mainly produced by activated T-cells, but is also produced by keratinocytes, NK-cells, mast cells, endothelial cells and monocytes. IL-3 promotes proliferation and differentiation of various hematopietic cell types, including pluripotent stem cells and more committed progenitors. IL-3 also affects activity of mature hematopoietic cells and often functions in combination with other cytokines. Human IL-3 is species-specific and shows no biological activity on murine cells.
• Source: Hordeum vulgare
• Species: Human
• Predicted N Terminal: His
• Form: Sterile filtered through a 0.2 μm filter. Lyophilized from PBS, pH 7.2
• Bio-activity: The bioactivity of recombinant human IL-3 was determined by its dose-dependent effects on the proliferation of TF-1 cells. The ED50 value for this effect is typically< 0.2 ng/ml, corresponding to a specific activity of>5 x 10e6 units/mg.
• Molecular Mass: Recombinant human IL-3 contains 133 amino acids and a 16 a.a. histidine-based tag for a total length of 149 a.a. and has a predicted molecular mass of 17.2 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 20-23 kDa in SDS-PAGE
• Endotoxin: Endotoxin level is less than 0.05EU/μg as measured by turbidimetric kinetic assay.
• Purity: >95% by SDS-PAGE gel analysis.
• Storage: The lyophilized protein is stable at room temperature for few weeks. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Gene Information

• Gene Name: IL3 interleukin 3 (colony-stimulating factor, multiple) [ Homo sapiens ]
• Official Symbol: IL3
• Synonyms: IL3; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; hematopoietic growth factor; IL 3; mast cell growth factor; MCGF; MGC79398; MGC79399; MULTI CSF; multilineage colony stimulating factor; P cell stimulating factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor; IL-3; MULTI-CSF
• Gene ID: 3562
• mRNA Refseq: NM_000588
• Protein Refseq: NP_000579
• MIM: 147740
• UniProt ID: P08700
• Chromosome Location: 5q23-q31
• Pathway: Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Calcium signaling in the CD4+ TCR pathway, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem
• Function: cytokine activity; growth factor activity; interleukin-3 receptor binding