Active Recombinant Human IL9,His-tagged

• Cat.No.: IL9-8866H
• Product Overview: Recombinant Human IL9 was produced in the endosperm tissue of barley grain (Hordeum vulgare).

Specification

• Description: IL-9 is a cytokine with immunoregulatory function, produced by activated T-helper type 2 lymphocytes. It acts on various cell types such as T- and B-lymphocytes, mast cells, haemopoietic progenitors and megakaryoblastic leukemia cell lines. IL-9 stimulates cell proliferation and prevents apoptosis. IL-9 may play a role in the pathogenesis of diseases such as certain lymphomas (Hodgin′s disease, anaplastic lymphoma) and asthma.
• Source: Hordeum vulgare
• Species: Human
• Predicted N Terminal: His
• Form: Sterile filtered through a 0.2 μm filter. Lyophilized from PBS
• Bio-activity: Bioactivity is assayed by measuring the dose dependent effect of human IL-9 on proliferation of murine TS1h9RA3 cell line, transfected with human IL-9 receptor (Renauld & Van Snick, Curr. Prot. in Immunol.2002). The ED50 of recombinant human IL-9 is 120 pg/ml.
• Molecular Mass: Recombinant human IL-9 contains 127 amino acids and a 16 a.a. histidine-based tag for a total length of 143 a.a. and has a predicted molecular mass of 16.2 kDa including his-tag. As a result of glycosylation, the recombinant protein migrates as 3 bands with an apparent molecular mass of 20, 22 and 24 kDa in SDS-PAGE.
• Endotoxin: Endotoxin level is less than 0.05EU/μg as measured by turbidimetric kinetic assay.
• Purity: >95% by SDS-PAGE gel analysis.
• Storage: The lyophilized protein is stable at room temperature for few weeks. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Gene Information

• Gene Name: IL9 interleukin 9 [ Homo sapiens ]
• Official Symbol: IL9
• Synonyms: IL9; interleukin 9; interleukin-9; homolog of mouse T cell and mast cell growth factor 40; HP40; IL 9; P40; p40 cytokine; p40 T cell and mast cell growth factor; T cell growth factor p40; cytokine P40; T-cell growth factor p40; p40 T-cell and mast cell growth factor; IL-9
• Gene ID: 3578
• mRNA Refseq: NM_000590
• Protein Refseq: NP_000581
• MIM: 146931
• UniProt ID: P15248
• Chromosome Location: 5q31-q35
• Pathway: Asthma, organism-specific biosystem; Asthma, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; IL-9 Signaling Pathway, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem
• Function: cytokine activity; growth factor activity; interleukin-9 receptor binding