Recombinant Human ATP, His-tagged

• Cat.No.: ATP6V1B2-10042H
• Product Overview: Recombinant Human ATPHis-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Description: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.
• Source: E.coli
• Species: Human
• Tag: His
• Protein length: 162-511a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: ATP6V1B2 ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 [ Homo sapiens ]
• Official Symbol: ATP6V1B2
• Synonyms: ATP6V1B2; ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2; ATP6B2, ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta polypeptide, 56/58kD, isoform 2 , ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 2 , VPP3; V-type proton ATPase subunit B, brain isoform; HO57; VATB; Vma2; V-ATPase B2 subunit; V-ATPase subunit B 2; vacuolar proton pump subunit B 2; H+ transporting two-sector ATPase; vacuolar H+-ATPase 56,000 subunit; endomembrane proton pump 58 kDa subunit; VPP3; ATP6B2; ATP6B1B2
• Gene ID: 526
• mRNA Refseq: NM_001693
• Protein Refseq: NP_001684
• MIM: 606939
• UniProt ID: P21281
• Chromosome Location: 8p21.3
• Pathway: Collecting duct acid secretion, organism-specific biosystem; Collecting duct acid secretion, conserved biosystem; Epithelial cell signaling in Helicobacter pylori infection, organism-specific biosystem; Epithelial cell signaling in Helicobacter pylori infection, conserved biosystem; Insulin receptor recycling, organism-specific biosystem; Iron uptake and transport, organism-specific biosystem; Metabolic pathways, organism-specific biosystem
• Function: ATP binding; hydrogen ion transmembrane transporter activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; hydrolase activity; hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances; proton