Recombinant Human CLDN16, GST-tagged

• Cat.No.: CLDN16-11288H
• Product Overview: Recombinant Human CLDN16 protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Description: Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is found primarily in the kidneys, specifically in the thick ascending limb of Henle, where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of magnesium ions. Defects in this gene are a cause of primary hypomagnesemia, which is characterized by massive renal magnesium wasting with hypomagnesemia and hypercalciuria, resulting in nephrocalcinosis and renal failure. This gene and the CLDN1 gene are clustered on chromosome 3q28.
• Source: E.coli
• Species: Human
• Tag: GST
• Protein length: 1-150a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: CLDN16 claudin 16 [ Homo sapiens ]
• Official Symbol: CLDN16
• Synonyms: CLDN16; claudin 16; claudin-16; HOMG3; hypomagnesemia 3; with hypercalciuria and nephrocalcinosis; paracellin 1; PCLN1; PCLN-1; paracellin-1; hypomagnesemia 3, with hypercalciuria and nephrocalcinosis
• Gene ID: 10686
• mRNA Refseq: NM_006580
• Protein Refseq: NP_006571
• MIM: 603959
• UniProt ID: Q9Y5I7
• Chromosome Location: 3q28
• Pathway: Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Cell-cell junction organization, organism-specific biosystem; Hepatitis C, organism-specific biosystem; Hepatitis C, conserved biosystem
• Function: identical protein binding; magnesium ion transmembrane transporter activity; structural molecule activity