||Recombinant Human ECE2 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.
||Endothelin-converting enzymes, such as ECE2 (EC 220.127.116.11), are type II metalloproteases that generate functionally pleiotropic members of the endothelin vasoactive peptide family (Lorenzo et al., 2001 [PubMed 11718899]).
||The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
||1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.