Active Recombinant Human METAP2, His-tagged

• Cat.No.: METAP2-635H
• Product Overview: Recombinant Human METAP2 (Ala2-Tyr478) fused with 10­His tag at C­terminal, was expressed in Spodoptera frugiperda.

Specification

• Cat. No.: METAP2-635H
• Description: This gene is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2.
• Source: Spodoptera frugiperda
• Molecular Mass: 54 kDa
• N-terminal SequenceAnalysis: Ala2
• Formulation: Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Activity: Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC. The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26. The specific activity is >15 pmol/min/µg, as measured using the described conditions.
• EndotoxinLevel: <1.0 EU per 1 μg of the protein by the LAL method.
• Storage: 6 months from date of receipt, ­70 °C as supplied. Avoid repeated freeze­thaw cycles.
• OfficialSymbol: METAP2

Gene Information

• Gene Name: METAP2 methionyl aminopeptidase 2 [ Homo sapiens ]
• Synonyms: METAP2; methionyl aminopeptidase 2; MAP 2; Peptidase M; eIF-2-associated p67 homolog; initiation factor 2-associated 67 kDa glycoprotein; metAP 2; methionine aminopeptidase 2; peptidase M 2; p67; MAP2; MNPEP; p67eIF2; EC 3.4.11.18
• Gene ID: 10988
• mRNA Refseq: NM_006838
• Protein Refseq: NP_006829
• MIM: 601870
• UniProt ID: P50579
• Chromosome Location: 12q22
• Function: aminopeptidase activity; metal ion binding; metalloexopeptidase activity