| Cat. No. : |
CD3E&D-310H |
| Description : |
T-cell surface glycoprotein CD3 delta/epsilon chain, also known as CD3D and CD3E respectively, are single-pass type I membrane proteins. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). The immunofluorescence defined that CD3 epsilon(CD3E0) was strongly stained bound to either CD 3 gamma or CD 3 delta. The recognition by this panel of anti-CD3 antibodies of CD3-gamma/ epsilon and CD3-delta/ epsilon complexes and not of CD3-epsilon alone was assessed by immunoprecipitation. |
| Source : |
Human Cells |
| Molecular Mass : |
The recombinant heterodimer of human CD3D/CD3E comprises 670 (333+354) amino acids and has a calculated molecular mass of 77.5 (37.5+40) KDa. As a result of glycosylation, the apparent molecular mass of human CD3D/CD3E heterodimer is approximately 45-50 KDa in SDS-PAGE under reducing conditions. |
| Predicted N terminal : |
Phe 22 & Asp 23 |
| Purity : |
> 90 % as determined by SDS-PAGE. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Formulation : |
Lyophilized from sterile PBS, pH 7.4. |
| Storage : |
Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
A hardcopy of COA with reconstitution instruction is sent along with the products. |
| Synonyms : |
CD3e molecule, epsilon (CD3-TCR complex); T3E; TCRE; FLJ18683; CD3E; T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3; CD3E antigen, epsilon polypeptide; CD3e antigen; CD3d molecule, delta (CD3-TCR complex); T3D; CD3-DELTA; CD3D; T-cell surface glycoprotein CD3 delta chain; OKT3, delta chain; CD3 antigen, delta subunit; T-cell receptor T3 delta chain; CD3d antigen, delta polypeptide (TiT3 complex); CD3d antigen |
