Recombinant Human HS3ST1, His-tagged

• Cat.No.: HS3ST1-2320H
• Product Overview: Recombinant human HS3ST1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: Heparan sulfate glucosamine 3-O-sulfotransferase 1, also known as HS3ST1, is sulfotransferase that utilizes 3"-phospho-5"-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. This protein catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT
• Molecular Mass: 36.2 kDa
• Protein length: 310aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSRPAELGQ QELLRKAGTL QDDVRDGVAP NGSAQQLPQT IIIGVRKGGT RALLEMLSLH PDVAAAENEV HFFDWEEHYS HGLGWYLSQM PFSWPHQLTV EKTPAYFTSP KVPERVYSMN PSIRLLLILR DPSERVLSDY TQVFYNHMQK HKPYPSIEEF LVRDGRLNVD YKALNRSLYH VHMQNWLRFF PLRHIHIVDG DRLIRDPFPE IQKVERFLKL SPQINASNFY FNKTKGFYCL RDSGRDRCLH ESKGRAHPQV DPKLLNKLHE YFHEPNKKFF ELVGRTFDWH
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml

Gene Information

• Gene Name: HS3ST1 heparan sulfate (glucosamine) 3-O-sulfotransferase 1 [ Homo sapiens ]
• Official Symbol: HS3ST1
• Synonyms: HS3ST1; heparan sulfate (glucosamine) 3-O-sulfotransferase 1; heparan sulfate glucosamine 3-O-sulfotransferase 1; 3OST1; 3-OST-1; h3-OST-1; heparan sulfate 3-O-sulfotransferase 1; heparin-glucosamine 3-O-sulfotransferase; heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1; 3OST
• Gene ID: 9957
• mRNA Refseq: NM_005114
• Protein Refseq: NP_005105
• MIM: 603244
• UniProt ID: O14792
• Chromosome Location: 4p16
• Pathway: Glycosaminoglycan biosynthesis - heparan sulfate, organism-specific biosystem; Glycosaminoglycan biosynthesis - heparan sulfate, conserved biosystem; heparan sulfate biosynthesis, organism-specific biosystem; heparan sulfate biosynthesis, conserved biosystem; heparan sulfate biosynthesis (late stages), organism-specific biosystem; heparan sulfate biosynthesis (late stages), conserved biosystem
• Function: [heparan sulfate]-glucosamine 3-sulfotransferase 1 activity; sulfotransferase activity; transferase activity