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Recombinant Human ATP1B1 protein, His-tagged

Cat.No.: ATP1B1-2279H
Product Overview: Recombinant Human ATP1B1(63 - 303aa) fused with His tag at N-terminal was expressed in E. coli.
Description: ATP1B1 belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. ATP1B1 is a beta 1 subunit.
Source: E. coli
Species: Human
Tag: His
Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea
Molecular Mass: 30.4kDa (264aa)
AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSEFKPTYQ DRVAPPGLTQ IPQIQKTEIS FRPNDPKSYE AYVLNIVRFL EKYKDSAQRD DMIFEDCGDVPSEPKERGDF NHERGERKVC RFKLEWLGNC SGLNDETYGY KEGKPCIIIK LNRVLGFKPK PPKNESLETY PVMKYNPNVL PVQCTGKRDEDKDKVGNVEY FGLGNSPGFP LQYYPYYGKL LQPKYLQPLL AVQFTNLTMD TEIRIECKAY GENIGYSEKD RFQGRFDVKI EVKS
Purity: > 90% by SDS - PAGE
Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration: 1.0 mg/ml (determined by Bradford assay)
Gene Name: ATP1B1 ATPase, Na+/K+ transporting, beta 1 polypeptide [ Homo sapiens ]
Official Symbol: ATP1B1
Synonyms: ATP1B1; ATPase, Na+/K+ transporting, beta 1 polypeptide; ATP1B; sodium/potassium-transporting ATPase subunit beta-1; adenosinetriphosphatase; Na, K-ATPase beta-1 polypeptide; Beta 1-subunit of Na(+),K(+)-ATPase; sodium/potassium-dependent ATPase beta-1 subunit; sodium/potassium-transporting ATPase beta-1 chain; MGC1798;
Gene ID: 481
mRNA Refseq: NM_001677
Protein Refseq: NP_001668
MIM: 182330
UniProt ID: P05026
Chromosome Location: 1q22-q25
Pathway: Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; Basigin interactions, organism-specific biosystem; Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Carbohydrate digestion and absorption, organism-specific biosystem;
Function: protein binding; sodium:potassium-exchanging ATPase activity;

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