Recombinant Human HDAC3, His-tagged

• Cat.No.: HDAC3-390H
• Product Overview: Recombinant Human HDAC3 was expressed in insect cell.

Specification

• Description: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
• Source: insect cells
• Species: Human
• Tag: His
• Form: 103 μg/μl in 50 mM Tris, pH 8.0, 138 mM NaCl and 10% glycerol.
• Specific activity: >1 U/µg.
• Molecular Mass: 49 kDa
• Protein length: 428 amino acids
• Purity: ~50% by SDS-PAGE.
• Applications: Study HDAC3 kinetics and inhibitor sensitivity and also the effect of the enzyme in transcriptional regulation, cell cycle progression, and oncogenesis.

Gene Information

• Gene Name: HDAC3 histone deacetylase 3 [ Homo sapiens (human) ]
• Official Symbol: HDAC3
• Synonyms: HDAC3; histone deacetylase 3; HD3; RPD3; RPD3-2; histone deacetylase 3; SMAP45; EC 3.5.1.98
• Gene ID: 8841
• mRNA Refseq: NM_003883
• Protein Refseq: NP_003874
• MIM: 605166
• UniProt ID: O15379
• Chromosome Location: 5q31
• Pathway: Alcoholism; BMAL1:CLOCK/NPAS2 Activates Circadian Expression; Cell cycle; Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants
• Function: chromatin DNA binding; enzyme binding; histone deacetylase activity; protein binding