Recombinant human ARPP19, His-tagged

• Cat.No.: ARPP19-2449H
• Product Overview: Recombinant human ARPP19 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: cAMP-regulated phosphoprotein 19, also known as ARPP19, is protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-62 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. This protein may indirectly enhance GAP-43 expression.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol
• Molecular Mass: 14.7kDa (135aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSAEVPE AASAEEQKEM EDKVTSPEKA EEAKLKARYP HLGQKPGGSD FLRKRLQKGQ KYFDSGDYNM AKAKMKNKQL PTAAPDKTEV TGDHIPTPQD LPQRKPSLVA SKLAG
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by BCA assay)

Gene Information

• Gene Name: ARPP19?cAMP-regulated phosphoprotein, 19kDa [?Homo sapiens(human) ]
• Official Symbol: ARPP19
• Synonyms: ARPP19; ENSAL; ARPP16; ARPP-16; ARPP-19; cAMP-regulated phosphoprotein, 19kDa; cAMP-regulated phosphoprotein 19; cyclic AMP phosphoprotein, 19 kD
• Gene ID: 10776
• mRNA Refseq: NM_006628
• Protein Refseq: NP_006619
• MIM: 605487
• UniProt ID: P56211
• Chromosome Location: 15q21.2
• Pathway: Cell Cycle; M Phase; MASTL Facilitates Mitotic Progression; Mitotic Prophase
• Function: phosphatase inhibitor activity; potassium channel regulator activity; protein phosphatase 2A binding; protein phosphatase inhibitor activity