||The protein was expressed as gst-tagged fusion protein by E.Coli and puri?ed by GSH-sepharose.
||Protein melan-A also known as melanoma antigen recognized by T-cells 1 orMART-1 is a proteinthat in humans is encoded by the MLANAgene. A fragment of the protein, usually consisting of the nine amino acids 27 to 35, is bound by MHC class Icomplexes which present it to T cells of the immune system. These complexes can be found on the surface ofmelanoma cells. Decameric peptides (26-35) are being investigated as cancer vaccines.
||1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.
||The protein is stored in PBS bu?er at -20 oC. Repeated freeze thaw cycles should be avoided.