Recombinant Human CKM, His tagged

• Cat.No.: CKM-116H
• Product Overview: Recombinant Human CKM (AAH07462.1) (Met1-Lys381), fused with a polyhistide tag at the N-terminus, was produced in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Fc&His
• Protein Length: 1-381 a.a.
• Predicted N Terminal: His
• Form: Lyophilized from sterile 100mM HEPES, pH 7.0.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant human CKM consists of 396 amino acids and predicts a molecular mass of 44.9 KDa. It migrates as an approximately 45 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CKM creatine kinase, muscle [ Homo sapiens ]
• Official Symbol: CKM
• Gene ID: 1158
• mRNA Refseq: NM_001824
• Protein Refseq: NP_001815
• MIM: 123310
• UniProt ID: P06732
• Chromosome Location: 19q13.32