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Recombinant Mouse Vcl, His tagged

Cat.No.: VCL-222M
Product Overview: Recombinant Mouse Vcl (NP_033528.3) (Met1-Gln1066), fused with a C-terminal polyhistidine tag, was produced in Human Cells.
Source: Human Cells
Species: Mouse
Tag: His
Predicted N Terminal: Met
Form: Lyophilized from sterile PBS, pH 7.4.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
Molecular Mass: The recombinant mouse VCL comprises 1077 amino acids and has a predicted molecular mass of 118.2 kDa. The apparent molecular mass of the protein is approximately 118 kDa in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE
Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4oC before opening to recover the entire contents.
Gene Name: Vcl vinculin [ Mus musculus ]
Official Symbol: Vcl
Gene ID: 22330
mRNA Refseq: NM_009502
Protein Refseq: NP_033528

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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