| Source : |
Insect Cells |
| Species : |
Mouse |
| Tag : |
His |
| Form : |
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.5.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL4-His at 10 μg/mL (100 μl/well) can bind rat IL4R-Fc, The EC50 of rat IL4R-Fc is 0.12-0.29 μg/mL.2. Measured by its ability to inhibit mIL4-dependent proliferation of HT-2 cells. The ED50 for this effect is typically 2-10 ng/ml in the presence of 2 ng/ml Recombinant Mouse IL4. |
| Molecular Mass : |
The recombinant mouse IL4 consists of 131 amino acids and predicts a molecular mass of 14.9 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions due to glycosylation. |
| Protein Length : |
Met 1-Ser140, Q136D, Y139D |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : |
> 95 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
