Recombinant Human DARS protein(Met1-Pro501), His-tagged

• Cat.No.: DARS-564H
• Product Overview: Recombinant Human DARS (P14868) (Met1-Pro501) was expressed in E. coli with a polyhistide tag at the N-terminus.

Specification

• Source: E. coli
• Species: Human
• Tag: N-His
• Protein length: Met1-Pro501
• Form: Lyophilized from sterile 50mM Tris, 100mM Nacl, 10% glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human DARS consists of 516 amino acids and predicts a molecular mass of 59 KDa. It migrates as an approximately 47 KDa band in SDS-PAGE under reducing conditions.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DARS aspartyl-tRNA synthetase [ Homo sapiens ]
• Official Symbol: DARS
• Synonyms: DARS; aspartyl-tRNA synthetase; aspartate--tRNA ligase, cytoplasmic; aspartate tRNA ligase 1; cytoplasmic; aspRS; aspartate tRNA ligase 1, cytoplasmic; aspartyl-tRNA synthetase, cytoplasmic; cell proliferation-inducing protein 40; cell proliferation-inducing gene 40 protein; MGC111579; DKFZp781B11202
• Gene ID: 1615
• mRNA Refseq: NM_001349
• Protein Refseq: NP_001340
• MIM: 603084
• UniProt ID: P14868